簡(jiǎn)介
OX1LP溶解氧測量?jì)x使用極譜溶解氧電極，可用于測量水相中的光合作用和/或呼吸作用，適用于藻類(lèi)、植物、動(dòng)物和細胞器（葉綠體和線(xiàn)粒體）的懸浮液。還可用于監測在水相中產(chǎn)生或消耗氧氣的化學(xué)和生化反應。溶解氧包配有儀器、附件、軟件和接口。
可提供從100ul（流通）到50ml不同體積的DO電極。水套反應杯內的氧電極可用于測量生物樣品或酶反應的O2消耗（或產(chǎn)生）。
采用簡(jiǎn)單的兩點(diǎn)校準， 可在相同的溫度下進(jìn)行校準和實(shí)驗，或者可以在軟件中對不同溫度下的測量進(jìn)行簡(jiǎn)單的溫度校正。隨機附校準所需的所有附件。
測量對象 v 藻類(lèi)光合作用與呼吸作用 v 細菌呼吸 v 微生物呼吸 v 小型水生動(dòng)物的呼吸 v 水生昆蟲(chóng)和幼蟲(chóng)呼吸 v 線(xiàn)粒體呼吸 v 葉綠體O2的產(chǎn)生 v 細胞懸浮呼吸 v 植物細胞懸浮光合作用 | 應用領(lǐng)域 v 水生植物光合氧代謝的測定 v 抑制劑對線(xiàn)粒體電子傳遞的影響 v 光依賴(lài)性葉綠體電子輸運分析 v 溫度對水生動(dòng)物攝氧量的影響 v 酶動(dòng)力學(xué)耗氧研究 |
基本組件 溶解氧反應杯電極（1,2.5,4,6,30,50ml） 反應杯電極放大器 磁力攪拌器 攪拌棒和O形圈 KCl填充液 膜材料和濾紙 水套用10英尺Excelon管 LabQuest迷你數據接口 Logger Pro軟件 定制安裝軟件 操作手冊 可選組件： 向反應杯中添加代謝物的注射器； 10微升，50微升，100微升 樣品溫度測量用熱敏電阻 校準LED光源 Q-Box堅固外殼，用于儲存和運輸所有部件 |
DO電極，帶磁力攪拌器和LED光源 |
不同體積的溶解氧反應杯電極 OX1LP-1 (1ml 有機玻璃) OX1LP-2.5 (2.5ml 玻璃 或有機玻璃) OX1LP-4 (4ml 有機玻璃) OX1LP-6 (6ml 玻璃) OX1LP-30 (30ml 有機玻璃) OX1LP-50 (50ml有機玻璃或玻璃) |
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軟件界面及數據
客戶(hù)評價(jià)
“I have tested my new Qubit system with isolated oyster mitochondria, and was extremely pleased with the results. I can’t believe I was suffering for the whole year with my old chamber. I have tried three different systems based on Clarke-type electrodes including this one, and this is by far the most convenient for use with small sample volumes and gives a very clean, repeatable trace with the best signal to noise ratio I ever had with micro-chambers.”
From Dr. Inna Sokolova, Assistant Professor, Biology Department, University of North Carolina at Charlotte
“我用分離的牡蠣線(xiàn)粒體測試了Qubit系統，對結果非常滿(mǎn)意。我真不敢相信忍受了整整一年舊培養室。我嘗試了三種不同類(lèi)型的克拉克電極，包括這一種，這是迄今為止測量小樣本很方便的系統，提供了一種非常干凈、可重復的軌跡，且在微型培養室中信噪比很好?！?/span>
——北卡羅來(lái)納大學(xué)生物系助理教授 Dr. Inna Sokolova
發(fā)表文獻
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· Croston, Tara L., et al. Functional deficiencies of subsarcolemmal mitochondria in the type 2 diabetic human heart. American Journal of Physiology-Heart and Circulatory Physiology 307.1 H54-H65 (2014)
· Sussarellu, Rossana, et al. Rapid mitochondrial adjustments in response to short-term hypoxia and re-oxygenation in the Pacific oyster, Crassostrea gigas.” The Journal of Experimental Biology 216.9 1561-1569 (2013).
· Dickinson, Gary H., et al. Environmental salinity modulates the effects of elevated CO2 levels on juvenile hard-shell clams, Mercenaria mercenaria. The Journal of experimental biology 216.14 2607-2618 (2013).
· Iris Bauer and Andreas Kappler. Rates and Extent of Reduction of Fe(III) Compounds and O2 by Humic Substances. Environ. Sci. Technol. Vol 43, Number 13, p4902–4908 ( 2009).
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· Bryce A. Mendelsohn, Bethany L. Kassebaum and Jonathan D. Gitlin. The zebrafish embryo as a dynamic model of anoxia tolerance. Developmental Dynamics Vol 237, Issue 7, p1780–1788 (2008).
· Bryce A. Mendelsohn and Jonathan D. Gitlin. Coordination of development and metabolism in the pre-midblastula transition zebrafish embryo. Developmental Dynamics Vol 237, Issue 7, p1789–1798(2008).
· Johnson EA, Rosenberg J, McCarty RE. Expression by Chlamydomonas reinhardtii of a chloroplast ATP synthease with polyhistidine-tagged beta subunits. Biochimica et Biophysica Acta 1767:374-380 (2007)
· Johnson E A. Altered expression of the chloroplasts ATP synthase through site-directed mutagenesis inChlamydamonas Reinhardtii. Photosynth Res vol 96:153-162 (2008)
· Charles W. Locuson, Peter M. Gannett and Timothy S. Tracy. Heteroactivator effects on the coupling and spin state equilibrium of CYP2C9. Archives of Biochemistry and Biophysics Vol 449, Issues 1-2, p115-129 (2006).
產(chǎn)地：加拿大
