可在野外現場(chǎng)同步測量P700（PS I）和葉綠素熒光（PS II）的儀器

光纖版雙通道調制葉綠素熒光儀DUAL-PAM/F最初是專(zhuān)門(mén)為附著(zhù)樣品(如苔蘚，地衣，巖石附著(zhù)藻類(lèi))和不方便夾住的樣品(如仙人掌，果實(shí)等一些非葉片器官)而設計的測量系統。當然，它也可以用于測量植物葉片和完整葉綠體、類(lèi)囊體和藻類(lèi)懸浮液。

光纖版雙通道調制葉綠素熒光儀DUAL-PAM/F與標準版的雙通道葉綠素熒光儀DUAL-PAM-100在基本功能上差別不大。也可以同時(shí)測量光系統II(PSII)葉綠素熒光(Fluo)和光系統I(PSI)氧化還原導致的近紅外差示吸收(P700/P700+)。

光纖版雙通道調制葉綠素熒光儀DUAL-PAM/F設計更加緊湊，所有光學(xué)組件均集成在主機內部，主機內置蓄電池，可以攜帶至野外現場(chǎng)測量。

光纖版雙通道調制葉綠素熒光儀DUAL-PAM/F與標準版的雙通道葉綠素熒光儀DUAL-PAM-100基本原理一樣，發(fā)表的文獻可以互相參考。光合作用文獻數據庫收錄數量有1680多篇，近五年，每年都有近200篇文獻發(fā)表。發(fā)文質(zhì)量高，其中不乏Molecular Plant，Nature Plants，Nature Communications，The Plant Cell，PNAS，New Phytologist，Plant Physiology，The Plant Journal等植物學(xué)領(lǐng)域的專(zhuān)業(yè)高分雜志文章(詳見(jiàn)附錄)。

主要功能：

1. 單獨或同步測量活體植物葉片或葉綠體/類(lèi)囊體/藻類(lèi)懸浮液的葉綠素熒光和P700差示吸收

2. 單獨或同步測量?jì)蓚€(gè)光系統的誘導動(dòng)力學(xué)曲線(xiàn)（包括快相和慢相）

3. 單獨或同步測量?jì)蓚€(gè)光系統的快速光曲線(xiàn)和光響應曲線(xiàn)

4. 淬滅分析、暗馳豫分析

5. 典型的P700氧化還原曲線(xiàn)

6. 通過(guò)葉綠素熒光和/或P700的同步測量可獲知兩個(gè)光系統的電子傳遞動(dòng)力學(xué)、電子載體庫(PQ)的大小、圍繞PSI的環(huán)式電子傳遞動(dòng)力學(xué)等

7. 可編編輯腳本，自定義完成復雜的測量過(guò)程。

8. 所有動(dòng)力學(xué)曲線(xiàn)均可導出Excel，實(shí)現光合反應過(guò)程動(dòng)力學(xué)曲線(xiàn)再現

測量數據：

1. 葉綠素熒光(PSII)數據：Fo、Fm、F、Fm’、Fv/Fm、Y(II)、Fo’、F/Fm、Fm’/Fm、qP、qL、qN、NPQ、Y(NPQ)、Y(NO)、ETR(II)、F(I)、F(I/)/Fo、α、Ik、ETRmax、Post-Illumination、Qa_Decay、Poly_300ms等

2. P700(PSI)數據：P700、Pm、Pm’、P700ox、Y(I)、Y(ND)、Y(NA)、ETR(I)、P700+-Reduction、PQ Poolsize等。

3. 光適應葉夾(選配)：可以測量環(huán)境光強PAR和環(huán)境溫度Temp.

DUAL-PAM/F與DUAL-PAM-100的區別：

DUAL-PAM/F具備所有DUAL-PAM-100的葉綠素熒光和P700測量功能，包括誘導曲線(xiàn)、光響應曲線(xiàn)、淬滅分析、暗馳豫分析、快速誘導動(dòng)力學(xué)、編程測量等等，其主要區別如下：

DUAL-PAM/F 光源和檢測器內置，帶光纖，適合于野外（或室內）測量，但不能升級P515/535等其它測量功能	DUAL-PAM-100 光源和檢測器外置，適合于室內使用，野外不方便。 但能升級P515/535、NADPH等其它測量功能。

應用領(lǐng)域：

1. 光合作用光系統光能轉換效率，過(guò)剩激發(fā)能耗散，狀態(tài)轉換，光合控制等機制研究。

2. 電子傳遞速率，包括線(xiàn)性電子傳遞速率和環(huán)式電子傳遞速率，及系統間電子載體庫研究。

3. 人工光合作用和能源相關(guān)領(lǐng)域，如生物光伏研究

4. 其他與植物光合作用結構解析、復合體組裝，色素合成與降解，逆境脅迫與抗逆生理相關(guān)的植物學(xué)，植物生理學(xué)，分子生物學(xué)，合成生物學(xué)，農學(xué)，林學(xué)、園藝，水生生物學(xué)、環(huán)境科學(xué)等領(lǐng)域。

操作軟件界面：

可選配件：

光適應葉夾2030-B：設計用于光下快速測量Y(II)，同時(shí)測量環(huán)境光強PAR和溫度Temp。按下葉夾上的紅色按鈕可觸發(fā)一次飽和脈沖測量。

葉片測量支架DUAL-BA：設計用于植物葉片高通量測量。為了快速更換樣品，通過(guò)彈簧鋼片將葉片固定在光纖最前端。彈簧片的矩形彎曲由釹磁鐵固定，釹磁鐵位于固定光纖的適配器夾具側面。彈簧鋼片可以在導向縫中自由移動(dòng)。DUAL-BA包含一個(gè)帶有光纖導軌的桌面支架。

懸浮樣品池KS-2500和帶光纖支架的磁力攪拌器MKS-2500：懸浮樣品池KS-2500由不銹鋼內膽和聚甲醛外殼組成。它包括一個(gè)400μl的樣品室，樣品室頂部配有一個(gè)7 mm光纖適配器、樣品室斜側面有一個(gè)注射器入口、側面有兩個(gè)用于連接外部循環(huán)水浴以進(jìn)行溫度控制的噴嘴。MKS-2500配備了一個(gè)經(jīng)過(guò)特殊改進(jìn)的攪拌底座，用于居中固定KS-2500懸浮樣品池。MKS-2500磁力攪拌器配還包含有機玻璃基板和立桿，用于在樣品池頂部安懸掛光纖。

產(chǎn)地：德國WALZ

代表文獻

數據來(lái)源：光合作用文獻Endnote數據庫

原始數據來(lái)源：Google Scholar

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